Document 3185
 DOCN  M94A3185
 TI    Prevalence of HTLV-I/II antibodies among HIV-1-asymptomatic patients in
       Sao Paulo, Brazil.
 DT    9412
 AU    Casseb JS; Caterino-de-Araujo A; Duarte AJ; Inst. de Infect. Emilio
       Ribas, Sao Paulo, Brazil.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0152). Unique
       Identifier : AIDSLINE ICA10/94369390
 AB    OBJECTIVE: To determine the seroprevalence of HTLV-I/II antibodies in
       HIV-1-asymptomatic subjects, and identify the major route of
       contamination with these dual retroviruses. PATIENTS AND METHODS: A
       cohort of 123 HIV-1-infected individuals in ambulatorial accompaniment
       to study the natural HIV history at the Secondary Immunodefiencies
       Ambulatory, Immunology Division, Hospital das Clinicas, University of
       Sao Paulo; others 208 HIV-1-asymptomatic patients recently diagnosed as
       HIV-1-infected at the Center of Counselling to Sexual Transmitted
       Diseases and AIDS (COAS) of Sao Paulo city, and 60 HIV-1 seronegative
       subjects presenting some risk behaviour to acquire these retroviruses
       were analysed in this study. Serum samples obtained from individuals
       were tested for the presence of HTLV-I/II antibodies using two ELISA
       kits. (Retrotek-HTLV-I, Cellular Products, NY, USA and Hemobio
       anti-HTLV-I, Embrabio, SP, Br). Sera dually positive were confirmed by
       WB (Immunoblot WB anti-HTLV-I, Embrabio, SP, Br). Positive and
       indeterminated sera were tested to WB 2.3 HTLV-I and HTLV-II (Cellular
       Products Inc. NY, USA). The serological results obtained were analysed
       according to risk behaviour for acquiring retrovirus and CDC
       classification system, 1986. RESULTS: from a total of 331 HIV-1-infected
       sera tested, 25 (7.5%) resulted reactive for HTLV-I/II antibodies in
       both ELISA tests; 6 (1.8%) had WB positivity and 9 (2.4%) presented an
       indeterminated WB profile. On WB 2.3 analysis, 7 (1.6%) confirmed HTLV-I
       and 2(0.6%) HTLV-II infections. Among 60 HIV-I seronegative individuals,
       none showed HTLV antibodies reactivity Among 36 IVDA, 1 (2.8%) presented
       HTLV-I and 1 (2.8%) HTLV-II infection on WB 2.3 analysis, respectively.
       These results contrast with those obtained previously, when IVDA with
       AIDS presented at least 26.4% of HTLVs infections.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*COMPLICATIONS/  EPIDEMIOLOGY
       Brazil/EPIDEMIOLOGY  Cohort Studies  Enzyme-Linked Immunosorbent Assay
       Human  HIV Seronegativity  *HIV-1  HTLV-I Antibodies/*BLOOD  HTLV-I
       Infections/BLOOD/COMPLICATIONS/*EPIDEMIOLOGY  HTLV-II Antibodies/*BLOOD
       HTLV-II Infections/BLOOD/COMPLICATIONS/*EPIDEMIOLOGY  Prevalence
       Risk-Taking  Substance Abuse, Intravenous  Support, Non-U.S. Gov't
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).